R Pulmo And Mammalian Type I Fibrillar Collagen Structural Analysis
Electrophoretic Mobility Profile
SDS PAGE was used to characterize R. pulmo peptide chain composition. The basic structure of collagen type I is composed of three polypeptide -chains , termed the coil, which are wound around each other. Under reducing conditions, R. pulmo collagen chain number and size were found to be present in the expected 2:1 ratio of 1 to 2 monomeric), with and forms also being observed, and closely matched the SDS-PAGE profile of high-purity rat tail and bovine type I collagen samples, thus demonstrating very similar chain composition between type I mammalian and R. pulmo collagen .
Figure 1. R. pulmo jellyfish electrophoretic banding and amino acid composition. SDS-PAGE analysis of R. Pulmo, rat tail and bovine collagens. R. Pulmo collagen was loaded by volume: lane 1: 12 ul, lane 2: 10 ul lane 3: 6 ul and lane 4: 3 ul. Thirty microgram of rat tail and bovine collagen were used as controls. Samples of acid solubilized R. Pulmo-rat tail-bovine collagen were compared for their amino acid composition.
Amino Acid Composition
Fourier-Transform Infrared Spectroscopy
Figure 2. FTIR spectrum of collagen from R. pulmo jellyfish. Attenuated total reflection Fouriertransform infrared spectroscopy spectra reveal the collagen bands in R. Pulmo collagen, with collagen I specific Amide pattern.
Breast Cancer Patients Should Avoid High
When youre told that you have breast cancer, its natural to wonder what may have caused the disease. But no one knows the exact causes of breast cancer. Doctors seldom know why one woman develops breast cancer and another doesnt, and most women who have breast cancer will never be able to pinpoint an exact cause Among cancers, milk and dairy intake was inversely associated with colorectal cancer, bladder cancer, gastric cancer, and breast cancer, and not associated with risk of pancreatic cancer, ovarian. If you want to take a deep dive, Dr. Gonzalez masterfully dismantles the ketogenic diet for cancer in the lengthy article below. This is not a scientific rebuttal, quibbling over theories about Warburg, glycosis, cell respiration, and ATP, rather it is a thoughtful, well-reasoned reflection from a medical doctor who was in the trenches of nutritional cancer treatment for nearly three decades
Does Collagen Supplement Increase Weight
No. , a study published in the Journal of Clinical Endocrinology & Metabolism, found that the amount of collagen in your body is not related to your weight. The researchers found no difference in weight gain or loss between those who took collagen supplements and those taking a placebo. In fact, the researchers concluded that the results of this study suggest that there is no significant difference between the effects of a collagen-based supplement and a non-collagen-containing supplement on body weight. .
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Transwell Invasion And Single Cell Migration Assay
The Transwell invasion assay was performed as previously described . As for the single cell migration assay, MCF-10A and MDA-MB-231 cells were seeded into a 4 chamber glass bottom dish at the density of 2000 cell/cm2. About 4 h after seeding, the single cell migration was monitored by Nikon BioStation every 10 min for 10 h . In some experiments, the cells were pre-incubated with 1 integrin blocking antibody AIIB2 with the final concentration of 80 g/ml for 30 min, and then the Transwell invasion and single cell migration assay were performed in the presence of the blocking antibody.
Can You Take Excessive Collagen Peptides
Numerous points in nature comply with a J-shaped contour way too much or insufficient are both negative. The very same may be true for collagen supplements, yet sadly, theres no indication of what would comprise excessive. Theres additionally no evidence that collagen poisoning is a worry if you following dosing guidelines.
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Collagen 1 Promotes Bc Cell Survival
In order to determine whether the collagen 1 is involved in the survival of FCS-starved-MCF-7 and T-47D cells, we studied the cell mortality after seeding the cells on a 2D collagen 1 coating. We found a decrease in cell mortality in the presence of collagen 1 after 24 h and 48 h post-starvation with a greater effect at 48 h, when compared to plastic condition . Next, we analysed apoptosis in cells seeded or not on collagen 48 h post-starvation using double labelling Annexin V/IP. Figure Figure1B1B showed that apoptotic cell rate is reduced by collagen 1 in MCF-7 , and T-47D cells . Moreover, the collagen 1 reduced Bax expression level in both MCF-7 and T-47D cells without affecting that of Bcl2 . These data showed that in FCS-starvation condition, collagen 1 protected the cells against apoptosis.
Collagen 1 induced MCF-7 and T-47D cells survival under serum starvation
Can Supplements Prevent Breast Cancer From Coming Back
So far, theres no proof that any vitamin, mineral, or herbal supplement can treat breast cancer or prevent it from coming back.
To date, there are no studies which demonstrate that certain supplements will lower your risk of recurrence, Johnson says. They may help with symptoms, but there isnt enough research to support using them to prevent breast cancer.
But there are other things you can do to lower your risk of breast cancer coming back. Make these lifestyle changes:
- Eat a healthy diet.
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Could Dietary Supplements Boost Breast Cancer Risk
Some antioxidant supplements may offset the anticancer effects of chemotherapy.
Certain dietary supplements may increase breast cancer recurrence risk and hasten death, according to a study published in the Journal of Clinical Oncology. Multivitamins, however, appear to be safe.
While the link between supplement intake and breast cancer risk remains tenuous, some previous research has found that certain supplements may reduce the effectiveness of chemotherapy treatments. Antioxidants in particular break down the cancer-killing free radicals that chemotherapy produces, according to the American Institute for Cancer Research .
The studys researchers recruited 1,134 people receiving chemotherapy for breast cancer who had a high risk of recurrence and followed them for 15 years or until they died of the disease. The participants completed a questionnaire about their supplement intake twice: once upon enrollment in the study and again six months after the conclusion of treatment. In total, 18% reported taking an antioxidant during treatment and 44% reported taking at least one multivitamin during treatment.
Lead study author Christine B. Ambrosone, PhD, the chair of the Department of Cancer Prevention and Control at Roswell Park Comprehensive Cancer Center, offered a possible explanation for these results. When you just pluck out specific vitamins and minerals from foods, you are losing all the effects that are probably there when using these nutrients from food, she said.
Effects Of Collagen Density On Cancer Cells
Increased stiffness compared to healthy tissue is a characteristic of most solid tumors that render them detectable by palpation. The high stiffness of tumor tissue has been shown to correlate with increased deposition of collagen as well as increased crosslinking of collagen fibers .
The high ECM stiffness is not only a passive bystander of cancer, but can also affect and drive many stages of tumor progression from malignant transformation and increased metabolic adaptability to enhanced intravasation, facilitating metastasis . In healthy tissue, matrix stiffness also controls many important cellular functions, such as development and homeostasis . In vitro, substrate stiffness has been shown to affect naÃ¯ve mesenchymal stem cells, which when cultured on soft matrices, mimicking brain tissue, commit to a neuron-like lineage while when cultured on rigid matrices, mimicking collagenous bone, commit to an osteogenic lineage .
Indirectly, a stiff matrix is also capable of supporting tumor progression by favoring growth of endothelial cells and thereby stimulating angiogenesis . Additionally, matrix stiffening promotes the activity of the transcriptional co-activators Yes-associated protein and transcriptional coactivator with PDZ-binding motif , which in turn are required for CAF-induced matrix stiffening, creating a positive feedback loop further driving the cancer-promoting effects described above .
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May Improve Skin Health
Collagen is a major component of your skin.
It plays a role in strengthening skin, as well as in elasticity and hydration. As you age, your body produces less collagen, leading to dry skin and the formation of wrinkles .
However, several studies have shown that collagen peptides or supplements containing collagen may help slow the aging of your skin by reducing wrinkles and dryness.
One review of 11 studies focusing mostly on women found that taking 310 grams of collagen per day for an average of 69 days led to improvements in skin elasticity and hydration .
These supplements may work by stimulating your body to produce collagen on its own. Additionally, collagen supplements may promote the production of other proteins that help structure your skin, including elastin and fibrillin .
There are also many anecdotal claims that collagen supplements help prevent acne and other skin conditions, but these arent supported by scientific evidence.
Conventional Versus Hypofractionated Radiotherapy In Node Positive Breast Cancer
|The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.|
|Verified May 2016 by Mahmoud Ellithy, Ain Shams University. Recruitment status was: RecruitingFirst Posted : February 24, 2016Last Update Posted : June 1, 2016|
Breast cancer patients operated with modified radical mastectomy or breast conservative surgery will be randomized for either adjuvant conventional radiotherapy versus hypofractionated radiotherapy for chest wall and axilla or breast and axilla.
The patients will be recruited for one year and will be followed for 5 years by monitoring local recurrence, cosmetic outcomes, health economic perspectives, and arm lymph edema.
|Study Type :|
May Promote Heart Health
Researchers have theorized that collagen supplements may help reduce the risk of heart conditions.
Collagen provides structure to your arteries, the blood vessels that carry blood from your heart to the rest of your body. Without enough collagen, arteries may become less flexible and elastic .
This may lead to atherosclerosis, a disease characterized by the narrowing of your arteries. This condition may lead to heart attack and stroke .
In a 6-month study, 31 healthy adults took 16 grams of collagen daily. They had experienced a significant reduction in measures of artery stiffness from the studys beginning to its end .
Additionally, their levels of HDL cholesterol rose by an average of 6%. HDL is an important factor in the risk of heart conditions, including atherosclerosis (
Nevertheless, more studies on collagen supplements and heart health are needed.
Who Advises Cancer Patients To Ignore Commercial Advertisements
Kellman also advises cancer patients to ignore commercial advertisements that promote the use of various supplements and vitamins, especially those irresponsibly marketed as cancer treatments, urging patients instead to seek the advice of a naturopathic provider or other health care professional trained in working with cancer patients.
Uncovering Collagens Role In Breast Cancer Metastasis
The study by the Garvan researchers was aimed at better understanding the role of the 300 to 400 molecules typically present in the extracellular matrix, a meshwork that provides support to all tissues in the body. The investigators looked to better understand how the tumor matrix changes over time and soon focused on collagen XII, one of 28 collagen types found in the body. Collagen XII helps to organize other collagens and plays an important role in the 3D structure of the extracellular matrix.
Using an array of tumor tissue types from early preclinical mouse models through to late-stage cancer, the Garvan team was able to demonstrate how the molecular makeup of the extracellular matrix changed over the development of tumors and catalogued these findings. They found that as the tumors developed, among the changes of many other matrix molecules, the level of collagen XII was also increased.
To determine the clinical relevance of collagen XII in breast cancer patients, collagen XII expression was analyzed in RNAseq data from the Cancer Genome Atlas breast cancer cohort, which confirmed significant upregulation of collagen XII across all primary breast tumor types, including TNBC, relative to non-tumor tissue.
Future studies to the role of collagen XII will seeks to include more humor tumor samples, while also exploring potential therapeutic pathways.
It May Cause Kidney Stones
One big collagen side effect on kidneys is the possibility of developing kidney stones.
According to Schoenfeld, If a person has a history of calcium oxalate kidney stones, it is a good idea to take it slow when adding collagen to the diet, starting with no more than 5-6 grams per day .
One of the amino acids that collagen is rich in, hydroxyproline, can be converted into oxalate and excreted through the kidneys into the urine. Under the right circumstances, this could trigger a kidney stone to form. Always drink plenty of water when adding collagen to the diet.
It may take a beating to your kidneys, but there are rarely any collagen side effects on livers. So, yay?
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Tension Homeostasis And Tumor Progression
Force is essential for normal tissue-specific development, in which it regulates cell survival, growth and migration, and orchestrates tissue organization and function. Increased matrix cross-linking and ECM protein deposition or parallel reorientation of matrix collagen can stiffen tissue locally to alter surrounding cells growth or drive cell migration. Loss of tissue homeostasis and mechanoreciprocity is a hallmark of disease. Although much is known about biochemical pathways that direct cell behavior, by comparison, little is known about how force regulates cell fate and tissue phenotype. Two primary cellular mechanisms involved in tumor invasion and migration are cellular physical rearrangement and reorientation of collagen by traction forces generated by epithelial cells , the consequence of EMT and cellular catabolism of ECM by enzymatic cleavage of collagen . Both can be regulated by tissue tension. Here we take a multiscale approach to describe tension homeostasis changes present at tumorstroma interface, ranging from the molecular level and the cellular level to the structural level .
Collagen 1 Promotes Basal Calcium Influx And Regulates Cell Survival Through A Calcium
We have already reported that extracellular Ca2+ regulates breast cancer cell survival and proliferation , we examined whether the variation of extracellular Ca2+ concentrations could affect the collagen-dependent survival. The cell viability was analysed after 48 h FCS-starvation. As expected, in 1.8 mM o collagen 1 decreased cell mortality in MCF-7 and T-47D cells . However, it failed to decrease the cell mortality in low Ca2+ conditions in both cell lines . Indeed, under low calcium conditions, collagen 1-induced cell mortality was increased compared to physiologic calcium conditions in both cell lines . Then, we investigated whether collagen 1 induced Ca2+ entry that we analysed through Mn2+ quenching of fura-2 fluorescence. Addition of Mn2+ decreased the fura-2 fluorescence in a time-dependent manner revealing a Mn2+ influx through plasma membrane Ca2+ channels . Our results showed an increased Mn2+ quenching by 5.7 and 11.5 folds in MCF-7 and 1.74 and 2.02 folds in T47-D cells cultured on collagen 1 for 24 h and 48 h post-starvation respectively . Altogether, our results showed that the collagen 1 promotes BC cell survival through a Ca2+-dependent pathway.
Collagen 1 promotes calcium influx and regulates cell survival by a calcium-dependent mechanism
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Can Collagen Supplements Cause Breast Growth
can collagen supplements cause breast growth?
The answer is yes. , a breast cancer researcher at the University of California, San Francisco, has found that the supplement can cause mammary gland growth. The study, published in the journal Breast Cancer Research, found a correlation between the amount of collagen in a supplement and the growth of breast tissue. In other words, the more collagen a woman has in her body, she will have more breast cells.The study also found the same correlation in women who took a collagen supplement. However, its not clear if the collagen is the cause of the breast cell growth or if it is just a side effect of taking the supplements.
Can Shoulder Blade Pain Be A Symptom Of Breast Cancer
Pain is not a common symptom of breast cancer. However, breast cancer that has metastasized can sometimes cause shoulder blade pain.
One of the most common locations for breast cancer metastasis is in the bones. Pain can be related to the cancer itself or fractures that happen when bones weaken.
Bladder, prostate, thyroid, uterus, lung, and kidney cancers can also spread to your bones. Lung and kidney cancers are also known to spread to the shoulder joint and shoulder blade.
It is also possible that pain near your right shoulder could be an indication that breast cancer has spread to your liver, because some of the nerve networks around the liver originate from the same nerves that attach near your right shoulder blade.
If youve had breast cancer in the past, and youre now experiencing shoulder pain, its a good idea to see a doctor about it as soon as possible. If it is a cancer relapse, treatment may help slow the cancers growth, relieve your symptoms, and protect your bones.
Yes. If youve had chemotherapy, radiation, or surgery, you may have pain around your shoulder blade because of those treatments.
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Does Low Estrogen Affect Collagen
In estrogen deficient women skin thickness is reduced by 1.13% and collagen content by 2% per postmenopausal year. Type I and III skin collagen is thought to decrease by as much as 30% in the first five years after menopause,27,28 which parallels the reduction in bone mass observed in post-menopausal women.