R Pulmo And Mammalian Type I Fibrillar Collagen Structural Analysis
Electrophoretic Mobility Profile
SDS PAGE was used to characterize R. pulmo peptide chain composition. The basic structure of collagen type I is composed of three polypeptide -chains , termed the coil, which are wound around each other. Under reducing conditions, R. pulmo collagen chain number and size were found to be present in the expected 2:1 ratio of 1 to 2 monomeric), with and forms also being observed, and closely matched the SDS-PAGE profile of high-purity rat tail and bovine type I collagen samples, thus demonstrating very similar chain composition between type I mammalian and R. pulmo collagen .
Figure 1. R. pulmo jellyfish electrophoretic banding and amino acid composition. SDS-PAGE analysis of R. Pulmo, rat tail and bovine collagens. R. Pulmo collagen was loaded by volume: lane 1: 12 ul, lane 2: 10 ul lane 3: 6 ul and lane 4: 3 ul. Thirty microgram of rat tail and bovine collagen were used as controls. Samples of acid solubilized R. Pulmo-rat tail-bovine collagen were compared for their amino acid composition.
Amino Acid Composition
Fourier-Transform Infrared Spectroscopy
Figure 2. FTIR spectrum of collagen from R. pulmo jellyfish. Attenuated total reflection Fouriertransform infrared spectroscopy spectra reveal the collagen bands in R. Pulmo collagen, with collagen I specific Amide pattern.
Vitamins And Minerals In Supplements
Your doctor may recommend certain vitamins and supplements to help you stay healthy after treatment.
Calcium.Chemotherapy can weaken your bones. A calcium supplement with vitamin D, which helps your body absorb calcium, may protect your bones from changes due to treatment.
Multivitamin. A regular multivitamin may help you get essential vitamins and minerals to improve your overall health.
Probiotics. Probiotics help boost immunity, says Tara Scott, MD, a womens health specialist in Akron, OH. This may help you stay healthy after your treatment. Probiotics have anti-inflammatory effects, which may help protect against cancer. But there isnt enough research yet to know for sure.
Vitamin D. Experts say theres a link between low levels of vitamin D and breast cancer. Theres no evidence a vitamin D supplement will lower your risk of recurrence, but your doctor may recommend it for your overall health.
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Brestrogen Up To 2 Cups In 5
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Flow Cytometry Analysis Apoptosis
Poly-HEMA pre-coated dishes were used for detachment cell culture . MCF-10A and MDA-MB-231 cells were cultured in regular media with 0.5% methyl cellulose in suspension at a density of 30,000 cells per cm2 . Cells were cultured in suspended condition for 24 h. And then were collected for annexin V analysis as manufacturers instructions.
Breast Massage : Treat Yourself In The Breast Way
Breast massage can benefit anyone with a chest at any stage of life. It can be particularly beneficial for lactating women and folks with a family history of breast cancer .
Breast massage is also a major part of the Indian Ayurvedic system of natural medicine, which has been around for more than 2,000 years.
A warm oil massage called Abhyanga can involve massaging the breast tissue to release toxins. While there isnt much research around this style of breast massage, a 2011 study found that Abhyanga decreased subjective stress levels and lowered heart rate.
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Xenograft Experiment And In Vivo Colonization Experiments
For the xenograft experiment, 6-week old female SCID mice were randomly grouped and injected with 2×106 control or collagen XIII-silenced MDA-MB-231-luc-D3H2LN cells at 4th mammary fat pad. Tumors were measured with a caliper every other day. Tumor volume was estimated using the formula . Twenty five days after tumor cell implantation, the primary tumors are removed by surgery. To detect lung metastasis, bioluminescent images were taken at 3 weeks after primary tumor removal with in vivo imaging system .
Are There Any Vitamins Or Supplements That You Would Strongly Suggest A Person With Breast Cancer Doesnt Take
I advise against taking dietary supplements that may have strong effects on levels in the body. This includes DHEA, red clover and licorice. Any that is labeled to naturally support a womans hormones or as a natural option are also things that I advise against using for anyone with a history of breast cancer.
For women with breast cancer, whole soy foods are safe and healthy to include in the diet and several studies suggest women with breast cancer who eat soy foods have a lower risk of breast cancer compared with women who do not eat these foods. But concentrated soy supplements are not well studied, so I advise women to stick with foods tempeh, miso, tofu, edamame, soy milk and to avoid soy-based dietary supplements.
Also consider what types of cancer treatments you are receiving. Some dietary supplements may be fine for some patients, but may need to be avoided during treatment for other patients. For example, there is some evidence that the dietary supplements berberine and quercetin may interact with . For someone on taxane medications, these would need to be avoided. For a woman who is not taking these drugs, these supplements may be perfectly safe.
The most important thing is to discuss your use of dietary supplements with your doctor or . These people will know your full medical history and your planned course of treatment. They will be able to advise you best on which dietary supplements may or may not be safe for you to use.
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Discovery Helps Show How Breast Cancer Spreads
Collagen fiber alignment at the tumor boundary is predictive of prognosis. Fibers that tend to be perpendicular to the tumor surface encourage metastasis and indicate a poor prognosis. Fibers that run parallel to the tumor surface protect against cancer spreading. Tumors without DDR2 or SNAIL1 tend to show the protective parallel fiber alignment.
Researchers at Washington University School of Medicine in St. Louis have discovered why breast cancer patients with dense breasts are more likely than others to develop aggressive tumors that spread. The finding opens the door to drug treatments that prevent metastasis.
It has long been known that women with denser breasts are at higher risk for breast cancer. This greater density is caused by an excess of a structural protein called collagen.
We have shown how increased collagen in the breasts could increase the chances of breast tumors spreading and becoming more invasive, said Gregory D. Longmore, MD, professor of medicine. It doesnt explain why women with dense breasts get cancer in the first place. But once they do, the pathway that we describe is relevant in causing their cancers to be more aggressive and more likely to spread.
The results appeared online May 5 in Nature Cell Biology.
We had no idea DDR2 would do this, said Longmore, also a professor of cell biology and physiology. The functions of DDR2 are not well understood, and it has not been implicated in cancer and certainly not in breast cancer until now.
Interaction Between Collagen And Tumor Matrix Components
The complex interactions among collagen and matrix proteins within ECM cells contribute to cancer initiation and progression, as shown in Fig. .
Fig. 2
The complexity between collagen and the extracellular matrix. Multiple stromal cells and the extracellular matrix play important roles in stimulating or inhibiting collagen functions via different pathways in cancer progression. Collagen-rich extracellular matrices can bind to other molecules to form dense fibrosis, which induces an anoxic environment and changes the condition of new blood vessels. The behavior of cancer cells is also closely related to collagen. This process also affects the activity and localization of innate and adaptive immune cells
Fibroblasts are another important cell type in the ECM. CAFs cause various collagen deposits to develop into intratumoral fibrosis, resulting in cancer occurrence, differentiation, and invasion . These CAFs can even originate from adipose tissue-derived stromal cells . CAFs are classified as tumor-restraining, tumor-promoting, secretory, or ECM-remodeling cells , which also exhibit CAF functions via mutated genes , the secreted cytokines IL-1, TNF- and NF-B, inflammatory signals, epigenetic regulation, etc. . Cancer cells can invade collagen with fibroblasts, which are characterized by cell cycle phases and small cancer cell nest formation . Additionally, CAFs exert anticancer and cancer-induced effects to show dual influences for cancer progression .
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Increased Deposition And Cross
The ECM scaffold undergoes considerable structural changes during tumor progression, including increased deposition of fibronectin, proteoglycans and collagens I, III and IV, and enhanced matrix cross-linking . Increased ECM deposition and remodeling creates a reorganized microenvironment to promote tumor progression by destabilizing cell polarity and cellcell adhesion, and augmenting growth factor signaling . The progressive ECM remodeling produces typical morphological changes characterized by linearization of interstitial collagens at tumor invasion front, with significant impacts on tumor cell biology including gene expression, cell differentiation, proliferation, migration and responses to treatments .
Type IV collagen expression demonstrated by quantum dot-525 . a Abundant type IV collagen fragments stochastically distributed in tumor tissues. b Rich type IV collagen in tumour stroma aligning with tumor nests. c, d Different characteristics between HCC and live cirrhosis tissues. Red arrowheads show stiff type IV collagen at interface of liver cirrhosis and tumor nests. Red arrows indicate the linear invasion “highways” for tumor cells escape. Scale bar=50 m
Protease-dependent collagen cross-linking based on LOX
A Travel Agent Appointment
If 2022 is the year that youre all about earning some miles and getting stamps on your passport, while I know that Google makes us think that we dont need to hire people for much of anything anymore, that is simply not the truth. If you can get your hands on a really good travel agent, aside from the fact that they can get you some pretty good deals, theyre also able to handle all of the details of your trips and get you access to perks that you probably wouldnt know about otherwise.
Plus, going with a travel agent is super convenient because they can plan your vacations while you focus on other things. Travel + Leisure published an article last year entitled, 8 Amazing Black-owned Travel Companies to Know and Support. If they cant hook you up, they can probably locate someone who can.
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Can Taking Curcumin/turmeric Help Reduce Inflammation In A Breast Cancer Survivor Is It Safe To Take During Active Treatment How Much Should Be Taken Daily
Curcumin is being studied extensively for its potential uses in cancer care. Curcumin is one of the main substances found in turmeric, which is the bright orange spice commonly found in Indian curry dishes. Turmeric is a rhizome or root- like spice, and looks a lot like ginger in its raw form. Think of ginger, only orange in color!
Turmeric has a long history of safe use as a culinary spice, and even when eaten in large quantities on a regular basis, it appears to be well-tolerated. Curcumin is one active ingredient in turmeric, and curcumin is being studied for safety and for a wide variety of conditions and diseases.
Curcumin has documented and associated with osteoarthritis. This is one of the conditions for which the best research evidence exists. There are hints that curcumin may help with other health conditions, including eye and skin inflammation, rashes, heart disease, inflammatory bowel disease , diabetes, gum disease, stomach ulcers, irritable bowel syndrome and rheumatoid arthritis. At this time, the evidence is insufficient to draw any firm conclusions about whether curcumin really is effective for managing or treating these conditions. Its frustrating to hear this, but we are at the of more research is needed, before we can recommend curcumin for these health conditions.
Study Links Changes In Collagen To Worse Pancreatic Cancer Prognosis
- Date:
- University of Wisconsin-Madison
- Summary:
- The first evidence linking a disturbance of the most common protein in the body with a poor outcome in pancreatic cancer has been uncovered by a team of researchers.
A study in the current journal Oncotarget provides the first evidence linking a disturbance of the most common protein in the body with a poor outcome in pancreatic cancer.
The study reinforces growing evidence that collagen, which forms fibrous networks in skin, tendons and muscles, is intimately involved in several cancers, says the paper’s corresponding author, Kevin Eliceiri, director of the Laboratory for Optical and Computational Instrumentation at the University of Wisconsin-Madison.
For the study, the Wisconsin researchers examined surgical tissues from 114 pancreatic cancer patients and identified a particular rearrangement of collagen fibers surrounding the tumor as a “biomarker” of early death.
A similar rearrangement of collagen has also been found in breast cancer, head, neck, esophageal and colorectal cancers.
“Collagen is the most abundant protein in the body,” says Eliceiri. “It’s a beautiful molecule — wavy, with a fibrous nature. Without it we would be a sack of nothing. With this little molecule, the specific fiber organization really matters to metastasis.”
Understanding collagen’s role in cancer could have several uses, Weber says.
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I Think Of My Essential Oils As Supplements I’ve Been Told That Using A Topical Blend Of Frankincense Sandalwood And Lemon May Help Lower The Risk Of Recurrence What Are Your Thoughts On These Or Other Possibilities
Essential oils have been used in a variety of cultures for thousands of years, but they are fairly new in the cancer care arena. There are no studies that have examined how the use of essential oils may affect cancer risk, or risk of in people who have had the disease. Some companies that manufacture and sell essential oils claim that these products can prevent or beat cancer, but these claims are not supported by scientific evidence in humans.
These claims of effectiveness against cancer are based largely on what is known as anecdotal evidence, plus some laboratory studies in cells . Unfortunately, anecdotal reports by people cannot be verified, so there is no way to determine if the person making the report is an actual patient who had a positive experience with the essential oil. studies are an important starting point in cancer research, but they are not a good way to know if the being tested will actually work in humans. Cancer cells can behave very differently in a test tube than they would behave inside of a human body.
The study authors felt that the gynecomastia occurred because of the possible weak estrogenic and antiandrogenic activities of lavender and tea tree oils. Avoiding these two essential oils is recommended in patients with -dependent tumors.
Evolution Of Breast Cancer Treatment
Breast cancer is the most frequently occurring malignant disease and leading cause of cancer-related death in women in the world, accounting for 710 % of the whole body all kinds of malignant tumors, tending to occur in 40- to 60-year-old women before and after menopause. Chinese females have a lower incidence of breast cancer compared with their counterparts in Western countries. However, the incidence of breast cancer has increased steadily at an amazing rate over the past two decades , making breast cancer the most common and the fifth most common cancer for Chinese urban and rural females, respectively .
Technological advances over the last decade have stimulated interest in even less invasive treatment of patients with localized breast cancer. One of the most attractive image-guided ablation therapies is high-intensity focused ultrasound ablation. HIFU ablation is a noninvasive procedure, i.e., requires no probe insertion and utilizes focussed ultrasound energy to coagulate tissue . HIFU ablation offers a promising method for noninvasive treatment of benign or malignant breast tumors. The breast is an organ with an excellent soft-tissue window that is required for the ultrasound beam to reach the target volume furthermore, the breast can be easily immobilized .
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Sample Preparation And Tacs Quantification
Intraclass correlation coefficients were used to evaluate the intra-observer and inter-observer discordance of feature determination. To ensure reproducibility, inter-observer correlation coefficients of the TACSs determined by the three observers were calculated. To assess the intra-observer discordance, we randomly selected MPM images of 30 patients for TACS feature extraction. Each panelist performed the extraction for 10 patients and repeated the same steps a week later. The mean intra-observer and inter-observer agreement of the TACS feature extraction among three panelists were 0.921 and 0.857 . An ICC value of > 0.8 is considered as highly consistent.
I Would Like To Try Pure Aloe Juice As A Supplement But Am Concerned That There May Be Estrogen Produced By The Plant Is This Something To Worry About
Aloe juice is considered safe when taken in small doses, but you should be sure to use a product that is certified latex-free. The aloe plant produces two main substances gel and latex. The gel is generally considered safe to take orally, but the latex is not.
Aloe latex can irritate your stomach and intestines, and it has a strong effect, leading to in many people. Additionally, aloe latex can cause kidney and liver damage and electrolyte abnormalities, such as low potassium levels in the body. These are very serious side effects.
If you want to take aloe gel, start with a small does, such as an ounce per day. You want to make sure you dont have any negative reactions to aloe before you take more than this. There isnt any good evidence that aloe gel will have estrogenic effects in the body, and it may even help regulate metabolism favorably by lowering cholesterol levels in the body.
It turns out that cholesterol is a precursor to estrogen. That means the body uses cholesterol to manufacture estrogen. If you take aloe gel, it can pull cholesterol out of your gastrointestinal tract as it passes through you then excrete the excess cholesterol in your stool. But this effect is likely small, so aloe gel should not be used in place of other medications you are taking to lower cholesterol or block estrogen.
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