Analysis Of Human Biopsies
All human biopsies were obtained from the Lombardi Cancer Center Tumor Bank and the Department of Pathology at the Georgetown University Medical Center. Biopsies were analyzed and classified by the pathologists. Tumor biopsies were sectioned and then placed on glass slides. The slides were then heated at 56°C for 30 min. For specimens not in polyester wax, the slides were treated with xylene two times for 5 min each at room temperature. All slides were treated two times with 100% ethanol for 2 min each at room temperature and then two times with 95% ethanol for 2 min each at room temperature. After rinsing in running distilled H2O for 2 min, the slides were then treated with microwave fix in the microwave for 15 min at half-power and then 10 min at full power. The slides were then left to cool for 2 h at room temperature. For immunohistochemistry with anti-PBR primary antibodies, tissue sections were treated with a 30% H2O2:methanol mixture for 5 min at room temperature to neutralize endogenous peroxidase activity and then washed well with PBS. Primary antibody in 10% calf serum in PBS was added to sections at a concentration of 1:250 at room temperature for 1 h. Secondary antibody reactions using either HRP or FITC were carried out at concentrations of 1:500, as described above. For PBR fluorescent labeling with compound 4 , sections were treated with 1 × 107m compound 4 for 2 h at room temperature in a dark room and then viewed on an Olympus BH-2 fluorescent microscope.
The Chicken N Waffles Tribute
One of his most recent blunders was last month after he made controversial about PnB Rocks girlfriend after his passing.
On his Instagram, he posted himself eating chicken and waffles with the caption:
Long live PnB Rock.
PnB Rock was shot and killed at Roscoes Chicken N Waffles in Los Angeles although it may have been too soon, Kodak attempted to pay homage.
Xxl Magazine On Twitter: Kodak Black Shows Support For Breast
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- Descriptions: Kodak Black shows support for breast cancer survivors https://trib.al/nAxhhrO · 8:10 AM · Oct 4, 2017 ·SocialFlow · 35. Retweets.
- More : Kodak Black shows support for breast cancer survivors https://trib.al/nAxhhrO · 8:10 AM · Oct 4, 2017 ·SocialFlow · 35. Retweets.
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Increased Expression Of The Pbr Correlates With Increased Aggressive Phenotype In Human Breast Cancer Cell Lines
Initial studies performed in MCF-7 human breast cancer cells indicated extremely limited expression of PBR . The data generated were insufficient for further Scatchard analysis. Before dismissing our original hypothesis, we decided to examine expression of the receptor in eight other human breast cancer cell lines using the PBR-specific, high-affinity ligand PK 11195. The results from these experiments indicated that those cell lines with a more invasive and chemotactic potential, such as HS-578-T and MDA-231, display dramatically increased levels of PBR binding relative to nonaggressive cell lines such as ZR-75-1, T47D, and MCF-7 . Furthermore, the MCF-7 Adriamycin-resistant derivative cell line, ADR, which expresses medium invasive and chemotactic potential as well as intermediate levels of CD44, expressed 2040% PBR binding relative to the MDA-231 cell line . Scatchard analysis of PBR binding in the MDA-231 and ADR cell lines further shows each to have a Bmax of 8.7 ± 1.4 and 1.3 ± 0.23 pmol/mg protein, respectively . As mentioned previously, despite obtaining specific PK 11195 binding in MCF-7 cells, the low binding levels were inadequate for estimating binding characteristics using Scatchard plot analysis .
Kodak Black And Supa Cindy Make Fans Proud They Host Event For Breast Cancer Patients/survivors
Kodak Black and Supa Cindy hosted an event for breast cancer patients and survivors. The fans could not be prouder, and they made sure to show this online as well.
‘If its one thing #KodakBlack is going to do, it’s giving back to his community! Yesterday alongside radio personality, #SupaCindy they hosted an event at Ives Estates Park in Miami to spotlight over 20 women who are currently battling breast cancer or have beaten the disease. Women attending the event received wigs, cash gifts, and even a journal with a note from Kodak, according to @local10news,’ The Shade Room notes.
TSR continued and noted that Kodak spoke about the women at the event, saying, ‘I know theyll appreciate it. I want to show these women theyre queens, theyre strong, and theyre going to get through it. Pray with them. Were going to get through it together.’
TSR noted that Taressa Hall, who is currently fighting breast cancer, said the event meant a lot to her.
‘It makes me continue to fight. It encourages me to go through this journey, continue through this journey, and it encourages me to be a witness to other people that we all can survive,’ said Taressa.
One other follower posted: ‘That’s what I’m tryna tell ya’ll. Kodak is always tryna give back. Always tryna give back!’
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Kodak Black Honors And Donates Big To Survivors Of Breast Cancer
On Tuesday, Kodak, whose real name is Bill Kapri, says he wanted these ladies to feel special since some of them are still fighting cancer.
The Florida rapper spent the day honoring the women who battled breast cancer with gifts and celebrated their never ending fight.
Kodak told WPLG, I know theyll appreciate it. Show these women theyre queens, theyre strong, and theyre going to get through it. Pray with them, were going to get through it together.
According to NBC Miami, Kapri teamed with local radio host Supa Cindy to hand out wigs, cash, a personal journal and a note from Kodak himself to more than 20 women.
The gesture was well-received by the women in attendance, with many suggesting it encourages them to keep fighting.
Taressa Hall is a three-year breast cancer survivor and says she appreciates the attention survivors and fighters receive during the month of October.
It makes me continue to fight it encourages me to go through this journey. Continue this journey, and it encourages me to be a witness to other people. We all can survive and we all we get this together. Hall shared.
Check out a clip from the event below.
Supa Cindy shared on Instagram, Today was such a BLESSING!! Kodaks heart is SO HUGE and to help women who are such fighters felt INCREDIBLE.
Kodak Black Supports Breast Cancer
He a lil confused, but he got the spirit
#kodakblack | CANCEL CULTURE NEEDS TO CANCEL KODAK BLACK, KODAK IS DONE IN MY EYES! More Info. We Have got 11 pics about I Support Breast Cancer Kodak images, photos, pictures, backgrounds, and more. In such page, we additionally have number of images out there. Such as png, jpg, animated gifs, pic art, symbol, blackandwhite, pic, etc.
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Nuclear Transport Of Cholesterol
Nuclei were isolated from MDA-231 and MCF-7 as described by Elango et al.. Isolated nuclei were resuspended in 1 ml of ice-cold PBS. Cholesterol uptake in MDA-231 and MCF-7 nuclei was examined in the presence of 6.7 nmcholesterol and 3 g of nuclear protein for 60 min at 37°C . Samples were then centrifuged at 500 × g for 30 min, and pellets were washed in 500 ml of ice-cold PBS. After a second centrifugation at 500 × g for 30 min, 200 l of 1.0 n NaOH was then added to the pellets and incubated overnight at 37°C. After incubation, 200 l of 1.0n HCl was added, and samples were mixed vigorously. Three ml of scintillation cocktail were then added, and radioactivity was measured by liquid scintillation spectroscopy using a Wallac 1409 Liquid Scintillation Counter.
Kodak Black Donates Money & Gifts To Breast Cancer Survivors: Theyre Queens
Northeast Miami-Dade, FL With Breast Cancer Awareness Month coming to a close, Kodak Black spent his Tuesday giving back in the Northeast Miami-Dade area as he showered breast cancer survivors with gifts and celebrated their journey and fight.
Kodak Black teamed up with 99JAMZ radio host Supa Cindy and met with more than 20 women to hand out wigs, cash, a personal journal and a note from Kodak himself.
I know theyll appreciate it, Kodak Black told WPLG. Show these women theyre queens, theyre strong, theyre going to get through it. Pray with them, were going to get through it together.
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Cali Crip Cmac Says F Kodak Black & Sick Fetish For His Own Momma
TRUMP PARDONED LIL WAYNE AND KODAK BLACK !, If you enjoyed this video please like & subscribe! ======================================= My Aunts Breast Cancer . #KODK Eastman Kodak WILL BLOW UP IN THE FUTURE!, Stock Market Today, we are covering different ticker symbols in the Stock Market Today. Request a Stock from the Market Today ..
Celebrities For Breast Cancer Awareness
As Breast Cancer Awareness Month kicks off today, ESSENCE.com salutes those celebs who are crusading against it and those who have survived this deadly disease that claims more African-American womens lives than any other race.
Gabrielle Union, ambassador for Susan G. Komen for the Cures Circle of Promise, joins more than 25,000 participants, including 1,600 breast cancer survivors, at the Susan G. Komen New York City Race for the Cure in Central Park on September 13, 2009. The 5K run/walk events goal was to raise more than $6 million for breast cancer research and community outreach programs.
Actress and singer Queen Latifah struts her stuff for the fourth annual Revlon Run/Walk for Women on May 5, 2001, in New York City. The event raises funds for breast and ovarian cancer research.
The actress lends her support to Super Saturday 12 to Benefit Ovarian Cancer Research Fund hosted by InStyle magazine at Novas Ark Project on August 1, 2009, in Water Mill, New York.
Robinson has been a longtime supporter and hosted The Denise Roberts Breast Cancer Foundation on Wednesday, September 30.
Robin Roberts, co-anchor of ABCs Good Morning America and a breast cancer survivor, was on hand for the Stand Up To Cancer event at the Kodak Theatre on September 5, 2008, in Hollywood, California.
Actor and breast cancer survivor Richard Roundtree speaks at the Sixth Annual Take-A-Hike to benefit breast cancer research on October 13, 2001, in Agoura, California.
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Kodak Black Apologizes To Pnb Rocks Girlfriend
The death of PnB Rock had plenty of reactions.
Kodak Black shared some disturbing comments about PnB Rocks girlfriend after she was accused of sharing their location before he was shot.
The Super Gremlin rapper would later apologize to Stephanie Sibounheuang in an Instagram live.
I give my apologies to her, Kodak said. I hope she keeps her head up.
Kodak said he was angry at the moment of PnB Rocks death and blamed her because the internet was doing it.
Kodak would later give a tribute to PnB Rock at the 2022 BET Hip-Hop Awards.
Kodak Black Just Did The Unthinkable During Breast Cancer Month
How many years of experience do you have in radio? I’ve been in radio since 2013. What are three of your hobbies? Flying the airwaves of the DFW, entertaining a crowd, & discovering new tasty food spots. Where is your hometown? St. Louis, MO. What makes your personality unique? My diverse childhood experiences of life shaped my mind and skill set to be the sharpest & most competitive it can be. If you attended college, where did you attend? Grambling State University.@pskillzflow
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Leave it up to the Florida flame Kodak Black himself, to keep a open ear and a warm heart to the current month that were all recognizing the month. Check out what Kodak did for women who are battling breast cancer. Kodak always finds away to give back to the community or less fortunate. This is the same guy who donated a large amount of money to a school library. Also this is the same guy who passed out air conditions to his former neighborhood a couple months ago.
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Pbr Expression And Localization Correlates With Increased Aggressive Phenotype In Human Breast Tumor Biopsies
Before characterization of the receptor in the MDA-231 cells, we examined whether the differences seen in the expression and localization of PBR in human breast cancer cell lines reflects real differences found in human breast cancer or an artifact of the cell culture system. To this end, we examined the expression and localization of PBR in normal breast tissue procured by reductive mammoplasty and metastatic aggressive breast tumor biopsies. ,A shows HRP staining of the PBR antiserum used to detect the Mr 18,000 molecular weight protein in normal breast tissue. This section was counterstained with hematoxylin. A light immunostaining for PBR can be seen. The low staining is better seen in ,B in which the hematoxylin counterstain was omitted. These data were confirmed using a FITC-labeled secondary antibody . It should be noted that PBR appears to be localized in the ductal epithelium. ,D is the phase contrast image of the ,C. The level of expression and localization of the Mr 18,000 molecular weight PBR protein was further examined in normal human breast tissue using compound 4 . Incubation of the sections with unlabeled PK 11195 displaced the fluorescent labeling seen with compound 4 . Similarly, low levels of cytoplasmic expression were obtained in specimens from in situ and noninvasive breast tumors . These data were replicated in multiple sections taken from specimens from three separate individuals.
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Determination Of Dna Content With Hoechst 33258
MDA-231 cells were plated on six-well plates at a concentration of 150,000 cells/well in DMEM supplemented with 10% FBS. After 24 h, cells were washed two times with PBS, and the medium was changed to DMEM supplemented with 0.1% FBS. After an additional 24 h, cells were treated with either 0, 1010, 108, 106, and 104m PK 11195 for 48 h. After incubation, cells were again washed two times with PBS and then solubilized with 200 l of 0.2% SDS in ETN buffer for 15 min at 37°C. Determination of DNA content was accomplished with the Hoechst 33258 dye as described previously .
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Ways To Support Breast Cancer Awareness
In addition to wearing the classic pink ribbon, there are other ways you can show your support for breast cancer awareness. Here are 10 that may appeal to the techie in you.
When most people think about the colors of fall, they think of oranges, yellows, reds, and browns. But pink has been making more of an appearance lately, thanks to National Breast Cancer Awareness Month celebrated annually during the month of October. For the past 26 years, NBCAM has aimed to educate and empower both men and women about the dangers of breast cancer and the precautions women can take by catching it early with yearly screenings.
According to BreastCancer.org, about one in eight women in the U.S. will develop some form of breast cancer in her lifetime. Thanks to research and advances in technology, weve come a long way in terms of awareness and treatment over the last quarter of the century.
Things are a lot different now than they were back in 1985 when NBCAM began. The Web has become a great resource for education, support, and communication. Women can now use it as a means to seek support from other patients and survivors around the world and to find information to supplement the materials they receive from doctors. It has also helped the NBCAM organization gain awareness and help spread its message about breast health, early detection and survivorship.
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Fluorescent Microscopy With The Fluorescent Pbr Ligand Compound 4
MA-10, MDA-231, MCF-7, and ADR cells were grown on glass coverslips as described previously . Cells were then washed twice with sterile PBS and incubated for 45 min with 1 m compound 4, a fluorescent derivative of the PBR ligand FGIN-27, with or without a competing PBR ligand, FGIN-27, at a concentration of 100 m. After the incubation period, the cells were washed with PBS and examined by fluorescent microscopy using an Olympus BH-2 fluorescence microscope.
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Kodaks Heart Is In The Right Place
We always need a devil’s advocate.
I for one am PRO breast cancer
Ive never understood these awareness movements. We all knew breast cancer was a thing. Now we all buy shirts to prove we know about the thing. How does any of that do anything about the thing itself?
It is supposed to make more people get checked for cancer, because the sooner it is diagnosed the greater the chances for the patient. Most people realize they’re sick when it is already too late. Another thing is hospitals and research get more donations I think.
Basically it is a reminder about the disease.
It’s time Support and awareness in made . Sarcoma Bone cancer YELLOW / RIBBON .. rare and deadly.
Iam stage two Survivor